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KMID : 0191120080230020302
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Journal of Korean Medical Science
2008 Volume.23 No. 2 p.302 ~ p.306
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Expression of Neutrophil Gelatinase-Associated Lipocalin in Calcium-Induced Keratinocyte Differentiation
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Lee Jeung-Hoon
Seo Young-Joon
Park Jang-Kyu
Seo Eun-Young
Kim Chang-Duk
Lee Kyung-Moon
Lim Jong-Soon
Lee Sang-Keun
Kye Kyung-Chae
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Abstract
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In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium- induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.
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KEYWORD
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LCN2 Protein, Human, Keratinocytes
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